Ishihara T, Kato J, Kitamura K, Katoh F, Fujimoto S, Eto T
Production of adrenomedullin in human vascular endothelial
cells
16th Scientific Meeting of the International Society of Hypertension
ISH Abstract Book
(Jun) 16: 1996
This study investigates whether the vasodilator adrenomedullin (AM),
besides the adrenal medulla, is also produced in human vascular
endothelial cells (ECs). Immunoreactive (ir-) AM in extract of both
cultured human umbilical vein endothelial cells and conditioned media
was measured by radioimmunoassay, and ir-AM was analyzed by reverse
phase high performance liquid chromatography (RP-HPLC). AM mRNA
expression in the ECs was analyzed by Northern blot analysis. In
addition, the effect of dexamethasone (DEX) on the production of AM
was examined in these cells.
The cultured endothelial cells secreted ir-AM into the media and
measurable intracellular levels were found. Dexamethasone (P <
0.05) augmented the secretion of ir-AM from the cells without an
effect on the intracellular ir-AM level. An analysis of RP-HPLC showed
that a large part of intracellular and secreted ir-AM eluted at the
identical position to that of human AM. Northern blot analysis showed
not only a presence of AM mRNA in the ECs but also an increased
expression in the DEX-treated cells.
Thus, human endothelial cells of the umbilical vein produce
adrenomedullin and dexamethasone stimulates the synthesis of this
peptide. These findings suggest not only a role of adrenomedullin as a
local modulator of the vascular tone but also a possibility of
endothelial cells are an important source of circulating
adrenomedullin (Carmine Zoccali, M.D, Reggio Calabria,
Italy).
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16th Scientific Meeting of the International Society of Hypertension
H: Special problems :
Endocrine hypertension
H: Pathophysiology :
Endothelium, Nitric Oxide