Ishizaki M, Oikawa K, Miyashita E
Usefulness of endotoxin test for assessing CAPD peritonitis by gram-negative organisms
XVIth Annual CAPD Conference
Perit Dial Int Suppl 2 (Feb) 16:S40 1996

These investigators measured endotoxin levels in spent dialysate with the Limulus assay in 11 peritonitis episodes. 5/11 dialysate samples were endotoxin positive, and in each case the peritonitis was due to a gram negative infection. In the remaining 6 patients dialysate endotoxin samples were negative. Two had Streptococcus cultured from the fluid and were 4 culture-negative. Patients were all treated with appropriate protocols depending on the endotoxin results. All were treated successfully.

Comment: The usefulness of this approach is questionable. The authors at best saved 2 days of unnecessary second antibiotic before the microbiological tests were available. The Limulus assay for endotoxin is a relatively costly assay and I would not recommend it to replace microbiological cultures. The value of serial endotoxin tests to evaluate the success of therapy of gram negative peritonitis is not convincingly demonstrated in the abstract. (Stephen I. Vas, M.D.)

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XVIth Annual CAPD Conference
Basic peritoneal dialysis : Peritonitis and exit-site infection